standards > model/format > DOI:10.25504/FAIRsharing.qpyp5g
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ready Gating-ML

General Information
The Gating-ML specification represents a proposal on how to form unambiguous XML-based gate definitions that may be used independently as well as included as one of the components of ACS. The specification supports rectangular gates in n dimensions (i.e., from one-dimensional range gates up to n-dimensional hyper-rectangular regions), polygon gates in two (and more) dimensions, ellipsoid gates in n dimensions, decision tree structures, and Boolean collections of any of the types of gates. Gates can be uniquely identified and may be ordered into a hierarchical structure to describe a gating strategy. Gates may be applied on parameters as in list mode data files (e.g., FCS files) or on transformed parameters as described by any explicit parameter transformation.


Countries that developed this resource Canada

Taxonomic range


How to cite this record Gating-ML; Gating-ML; DOI:; Last edited: Jan. 8, 2019, 1:40 p.m.; Last accessed: Jan 26 2022 9:04 a.m.

Record updated: March 15, 2018, 10:20 a.m. by The FAIRsharing Team.

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Additional Information


    No tools defined


Access / Retrieve Data

Conditions of Use


Flow cytometry data standards.

Spidlen J,Shooshtari P,Kollmann TR,Brinkman RR
BMC Res Notes 2011

View Paper (PubMed) View Publication

ISAC's Gating-ML 2.0 data exchange standard for gating description.

Spidlen J,Moore W,Brinkman RR
Cytometry A 2015

View Paper (PubMed) View Publication

Gating-ML: XML-based gating descriptions in flow cytometry.

Spidlen J,Leif RC,Moore W,Roederer M,Brinkman RR
Cytometry A 2008

View Paper (PubMed) View Publication

Related Standards

Terminology Artifacts

No semantic standards defined

Models and Formats

Identifier Schemas

No identifier schema standards defined


No metrics standards defined

Related Databases (1)
FlowRepository is a database of flow cytometry experiments where you can query and download data collected and annotated according to the MIFlowCyt standard. Data are generally associated with peer reviewed manuscripts.

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Grant Number(s)

  • 1R01EB008400 (National Institute of Biomedical Imaging and Bioengineering (NIBIB), USA)

  • EB005034 (National Institute of Biomedical Imaging and Bioengineering (NIBIB), USA)

  • R01 EB008400 (U.S. National Library of Medicine)