standards > model/format > DOI:10.25504/FAIRsharing.a4wgws
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ready Real-time PCR Data Markup Language

Abbreviation: RDML

General Information
The RDML file format is developed by the RDML consortium ( and can be used free of charge. The RDML file format was created to encourage the exchange, publication, revision and re-analysis of raw qPCR data. The core of an RDML file is an experiment, not a PCR run. Therefore all the information is collected which is required to understand an experiment. The structure of the file format was inspired by a database structure. In the file are several master elements, which are then referred to in other parts of the file. This structure allows to reduce the amount of redundant information and encourages the user to provide useful information. The Real-time PCR Data Markup Language (RDML) is a structured and universal data standard for exchanging quantitative PCR (qPCR) data. The data standard should contain sufficient information to understand the experimental setup, re-analyse the data and interpret the results. The data standard is a compressed text file in Extensible Markup Language (XML) and enables transparent exchange of annotated qPCR data between instrument software and third-party data analysis packages, between colleagues and collaborators, and between authors, peer reviewers, journals and readers. To support the public acceptance of this standard, both an on-line RDML file generator is available for end users, as well as RDML software libraries to be used by software developers, enabling import and export of RDML data files.

How to cite this record RDML; Real-time PCR Data Markup Language; DOI:; Last edited: Jan. 8, 2019, 1:31 p.m.; Last accessed: Oct 24 2021 11:20 p.m.

This record is maintained by untergasser

Record updated: March 15, 2018, 10:14 a.m. by The FAIRsharing Team.

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Conditions of Use

Applies to: Data use


RDML: structured language and reporting guidelines for real-time quantitative PCR data.

Lefever S, Hellemans J, Pattyn F, Przybylski DR, Taylor C, Geurts R, Untergasser A, Vandesompele J
Nucleic Acids Res. 2009

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RDML-Ninja and RDMLdb for standardized exchange of qPCR data.

Ruijter JM,Lefever S,Anckaert J,Hellemans J,Pfaffl MW,Benes V,Bustin SA,Vandesompele J,Untergasser A
BMC Bioinformatics 2015

View Paper (PubMed) View Publication

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Grant Number(s)

  • 050-71-052 (The Netherlands Organisation for Health Research and Development (ZonMw), The Hague, The Netherlands)